This table indicates the grams of solid ammonium sulphate (at 25°C) to be added to one litre of solution to produce a desired change in the concentration of ammonium sulphate. A saturated solution is 4.1 M and requires 767 grams of salt per litre. This table is based on Green and Hughes in Methods Enzymol. 1, 67-90 (1955).
How to use ammonium sulphate concentration table:
Select your initial concentration of ammonium sulphate in percent from saturation from the left column, then select required final concentration of ammonium sulphate in percent from saturation from the top row. The intersection point will give you and exact amount of ammonium sulphate (AmSO4) in grams needed for 1 litre of initial solution.
%
|
10
|
15
|
20
|
25
|
30
|
33
|
35
|
40
|
45
|
50
|
55
|
60
|
65
|
70
|
75
|
80
|
85
|
90
|
95
|
100
|
0
|
56
|
84
|
114
|
144
|
176
|
196
|
209
|
243
|
277
|
313
|
351
|
390
|
430
|
472
|
516
|
561
|
610
|
662
|
713
|
767
|
10
|
|
28
|
57
|
86
|
118
|
137
|
190
|
183
|
216
|
251
|
288
|
326
|
365
|
406
|
449
|
494
|
540
|
592
|
640
|
694
|
15
|
|
28
|
57
|
88
|
107
|
120
|
153
|
185
|
220
|
256
|
294
|
333
|
373
|
415
|
459
|
506
|
556
|
605
|
657
|
20
|
|
29
|
59
|
78
|
91
|
123
|
155
|
189
|
225
|
262
|
300
|
340
|
382
|
424
|
471
|
520
|
569
|
619
|
25
|
|
30
|
49
|
61
|
93
|
125
|
158
|
193
|
230
|
267
|
307
|
348
|
390
|
436
|
485
|
533
|
583
|
30
|
|
19
|
30
|
62
|
94
|
127
|
162
|
198
|
235
|
273
|
314
|
356
|
401
|
449
|
496
|
546
|
33
|
|
12
|
43
|
74
|
107
|
142
|
177
|
214
|
252
|
292
|
333
|
378
|
426
|
472
|
522
|
35
|
|
31
|
63
|
94
|
129
|
164
|
200
|
238
|
278
|
319
|
364
|
411
|
457
|
506
|
40
|
|
31
|
63
|
97
|
132
|
168
|
205
|
245
|
285
|
328
|
375
|
420
|
469
|
45
|
|
32
|
65
|
99
|
134
|
171
|
210
|
250
|
293
|
339
|
383
|
431
|
50
|
|
33
|
66
|
101
|
137
|
176
|
214
|
256
|
302
|
345
|
392
|
55
|
|
33
|
67
|
103
|
141
|
179
|
220
|
264
|
307
|
353
|
60
|
|
34
|
69
|
105
|
143
|
183
|
227
|
269
|
314
|
65
|
|
34
|
70
|
107
|
147
|
190
|
232
|
275
|
70
|
|
35
|
72
|
110
|
153
|
194
|
237
|
75
|
|
36
|
74
|
115
|
155
|
198
|
80
|
|
38
|
77
|
117
|
157
|
85
|
|
39
|
77
|
118
|
90
|
|
38
|
77
|
95
|
|
39
|
|