- Description
- Specifications
Catalog #: PKA-246
Description:
Soluble TIE-1 Human Recombinant fused with the Fc part of human IgG1 produced in baculovirus is a homodimeric, glycosylated, polypeptide containing 749 amino acids and having a total molecular mass of 250 kDa. Human TIE-1/Fc monomer has a calculated molecular mass of approximately 105 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 125 kDa protein in SDS-PAGE under reducing conditions.
The TIE1 Fc Chimera is purified by proprietary chromatographic techniques.
Synonyms:
Tyrosine kinase with immunoglobulin-like and EGF-like domains 1, JTK14, TIE, TIE1.
Source:
Insect Cells.
Purity:
Greater than 90.0% as determined by(A)Analysis by RP-HPLC.
(B)Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized TIE-1 Fc Chimera in sterile water not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
TIE-1 Fc Chimera was lyophilized from a concentrated (1 mg/ml) sterile solution containing 20mM Tris, 0.5M NaCl, 10% Sucrose.
Stability:
Lyophilized sTIE-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution TIE-1 should be stored at 4 °C between 2-7 days and for future use below
-18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
