Ascites Production
While tissue culture is always the preferred antibody production methodology, some hybridomas only grow well in vivo. We offer ascites production in as few as 5 mice (or as many as you need).
We will need a frozen ampule of your hybridoma cells (or a 25 cm tissue culture flask of growing cells) to culture the required number of cells.
Day |
Procedure |
1 | Prime Balb/C mice with Pristane. |
7 | Inject 5 x 105 hybridoma cells per mouse |
14 | IP tap |
21 | IP tap |
28 | IP tap |