While tissue culture is always the preferred antibody production methodology, some hybridomas only grow well in vivo. We offer ascites production in as few as 5 mice (or as many as you need).

We will need a frozen ampule of your hybridoma cells (or a 25 cm tissue culture flask of growing cells) to culture the required number of cells.

Day

Procedure

1 Prime Balb/C mice with Pristane.
7 Inject 5 x 105 hybridoma cells per mouse
14 IP tap
21 IP tap
28 IP tap

 

 

 

 

 

 

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