Tissue culture method - It is possible to obtain sufficient amounts of monoclonal antibodies just by static culture in 6 or 24 well plates and it largely depends on the final application of the monoclonal antibody. If customers need larger amounts of monoclonal antibodies we generally use the roller bottles for large scale production. Any variation in cell density is not well tolerated by the hybridomas. We ensure that the cultures are gradually transferred into larger containers and have the right density before finally transferring to the roller bottles.

Ascites method: In this method the hybridomas are grown inside the peritoneal cavity of the mouse which is genetically compatible and is the same strain as the myeloma as well as the spleen donor. Generally this we use this procedure if a customer specifically requests it and also if the hybridoma is difficult to grow the invitro cell culture. Once the first mouse is acclimatized to growing in vivo conditions, it can be passaged from one mouse to the other. We also freeze some of these hybridomas from ascites for future use.