Denovo Biotechnology’s Real-Time PCR kits for the quantification of Herpesvirus copy numbers
Each kit contains a ready-to-use primer/probe mix consisting of forward and reverse primers combined with a FAM/BHQ-labeled probe specific for the target sequence. This probe mix is supplied at 20x concentration and may be used with standard real-time PCR reagents.
For absolute copy number calculations, a tube of standards is also included containing the target sequence. These standards may be diluted to create a standard curve.
For best results, we recommend our Hot Start Master Mix with pre-optimized amplification parameters.