Purer the antigen the better is the antibody response. We generally require up to 90% pure antigen for immunizing the animals and to get better results. Please avoid Additives such as PMSF, sodium azide etc that are toxic to animals. Low amounts of SDS, imidazole, urea, guanidine, non-ionic detergents, EDTA or EGTA and polyacrylamide are acceptable. Talk to us if you have any purity concerns.Antigen should be supplied in buffered (Phosphate, Tris, MOPS, Hepes) saline solution.