The relative affinity is the affinity exhibited by two antibodies towards the same antigen. This is done using ELISA method where the the relative affinity of up to two antibody samples is determined in duplicate using covalently immobilized antigen or peptide.  The affinity is expressed as a ratio of the (inverse) dilution at which the sample exhibited a 0.500 absorbance in the absence and presence of denaturant. The entire range of dilutions is plotted versus absorbance on the provided certificate of completion.

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