Description: Polyclonal antibody to recombinant glutathione-S-transferase (GST) was raised by immunizing rabbits with affinity purified GST. The protein was expressed in bacteria and purified by glutathione-affinity chromatography. The product is provided as crude serum with 0.05 % sodium azide as a preservative.
Type: Polyclonal Rabbit Antibody.
Specificity: Polyclonal antiserum against GST recognizes recombinant GST. It reacts specifically to a variety of GST fusion proteins used as targets in immunoblotting experiments. It also immunoprecipitates the target proteins efficiently. This antibody is reactive with immobilized native antigens on ELISA plates as well.
Storage: For continuous use, store at 2-8 0 °C. For extended storage freeze, in desirable aliquots. Repeated freezing and thawing is not recommended.
Dilution: Western Blotting; 1/2,000.
Usage: Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Usage: Polyclonal antibody against GST may be used for the capturing of GST fusion proteins using various immunochemical assays including immunobotting, dot blotting, immunoprecipitation, ELISA and immunohistochemistry (immunofluorescence or immuno-enzymatic staining).