- Description
- Specifications
Catalog #: PRO-1237
Description:
Protein G His Tag Recombinant produced in E.Coli is a 202 amino acids protein which contains amino acid 190-384 of the Streptococcus sp with a C-terminal 6-His tag, and having a molecular mass of 22.4kDa. But it migrates with an apparent molecular mass of 32kDa in SDS-PAGE.
The Protein G His Tag is purified by proprietary chromatographic techniques.
Source:
Escherichia Coli.
Applications:
Protein G binds to the constant region of many species of immunoglobulin G. It can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity.
Purity:
Greater than 95.0% as determined by SDS-PAGE and HPLC analyses.
Solubility:
It is recommended to reconstitute the lyophilized Protein G in sterile 18M?-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
Lyophilized with no additive.
Stability:
Lyophilized Protein G although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution Protein G should be stored at 4 °C between 2-7 days and for future use below -18 °C.
Please prevent freeze-thaw cycles.
Specificity:
1. Binds with greater affinity to most mammalian immunoglobulins than Protein A, including human IgG3 and rat IgG2a.
2. Does not bind to human IgM, IgD and IgA.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
