Catalog #: PKA-248
Soluble TIE-2 Human Recombinant fused with the Fc part of human IgG1 produced in baculovirus is a monomeric, glycosylated, polypeptide containing 730 amino acids and having a total molecular mass of 250 kDa. Human TIE-2/Fc monomer has a calculated molecular mass of approximately 125 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 140 kDa protein in SDS-PAGE under reducing conditions.
The TIE2 Fc Chimera is purified by proprietary chromatographic techniques.
Angiopoietin-1 receptor precursor, Tyrosine-protein kinase receptor TIE-2, hTIE2, Tyrosine-protein kinase receptor TEK, p140 TEK, Tunica interna endothelial cell kinase, CD202b, VMCM, VMCM1, TIE2.
Greater than 90.0% as determined by(a)Analysis by RP-HPLC.
(b)Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized TIE-2 Fc Chimera in sterile water not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
TIE-2 Fc Chimera was lyophilized from a concentrated (1 mg/ml) sterile solution containing 1x PBS.
Lyophilized sTIE-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution TIE-2 should be stored at 4 °C between 2-7 days and for future use below
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Measured in a functional ELISA assay. When TIE-2/Fc is immobilized at 4 µg/mL (100 µl/well), it binds rh Angiopoietin-2 with a linear range of 2 -100 ng/ml.
Sterile Filtered White lyophilized (freeze-dried) powder.
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.