- Description
- Specifications
Catalog #: CYT-248
Description:
The ANGPTL3 Human Recombinant is produced with N-terminal fusion of His-Tag.
The Angiopoietin-like protein 3 His Tagged Fusion Protein is 26kDa containing 207 amino acid residues of the ANGPTL3 Human and 16 additional amino acid residues – His-Tag (underlined).
MRGSHHHHHH GMASHMSRID QDNSSFDSLS PEPKSRFAML DDVKILANGL LQLGHGLKDF VHKTKGQIND IFQKLNIFDQ SFYDLSLQTS EIKEEEKELR RTTYKLQVKN EEVKNMSLEL NSKLESLLEE KILLQQKVKY LEEQLTNLIQ NQPETPEHPE VTSLKTFVEK QDNSIKDLLQ TVEDQYKQLN QQHSQIKEIE NQLRRTSIQE PTEISLSSKP RAP.
Synonyms:
Angiopoietin 5, ANGPT5, ANGPTL3, Angiopoietin Like Protein 3.
Source:
Escherichia Coli.
Applications:
Western blotting, ELISA.
Purification Method:
One-step procedure using affinity Ni-NTA chromatography.
Purity:
Angiopoietin 5 purity is greater than 95% as determined by SDS-PAGE.
Solubility:
Add 0.2 ml of 0.1M Acetate buffer pH-4 and let the lyophilized pellet of ANGPTL3 Human dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of Angiopoietin 5 is limited.
Formulation:
ANGPTL3 Human Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.
Stability:
Store lyophilized ANGPTL3 Human at -20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted Angiopoietin 5 can be stored at 4 °C for a limited period of time; it does not show any change after two weeks at 4 °C.
Specificity:
The amino acid sequence of the ANGPTL3 Human recombinant is 100% homologous to the 17-223 amino acid sequence of the Human Angiopoietin-like proteins-3 precursor without signal sequence.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
