Description: Polyclonal antibody to recombinant glutathione-S-transferase (GST) was raised by immunizing rabbits with affinity purified GST. The protein was expressed in bacteria and purified by glutathione-affinity chromatography. From the crude serum, antigen-specific antibodies were purified by antigen-affinity chromatography. The product is provided as antigen-affinity purified IgG, with 0.05 % sodium azide as a preservative.
Type: Polyclonal Rabbit Antibody.
Concentration: 1mg/1ml .
Specificity: The monospecific polyclonal IgG antibody against GST recognizes recombinant GST. It reacts specifically to a variety of GST fusion proteins used as targets in immunoblotting experiments. It also immunoprecipitates the target proteins efficiently. This antibody is reactive with immobilized native antigens on ELISA plates as well.
Note: The above dilution may vary with different GST fusion proteins. Therefore, the optimal working dilution may have to be determined for best results.
Storage: For continuous use, store at 2-8 °C. For extended storage freeze, in desirable aliuots. Repeated freezing and thawing is not recommended.
Dilution: Western Blotting: 1/10,000 ELISA: 1/200,000.
Usage: Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Usage: Polyclonal antibody against GST may be used for the capturing of GST fusion proteins using various immunochemical assays including immunobotting, dot blotting, immunoprecipitation, ELISA and immunohistochemistry (immunofluorescence or immuno-enzymatic staining).