- Description
- Specifications
Catalog #: ANT-181
Type:
Polyclonal Antibody.
Applications:
This polyclonal antibody can be used for detection of Anthrax LF protein in western blot.
Not tested in other applications.
Isotype:
Rabbit Ig.
Storage:
Store at 4 °C, stable for 6 months. For long-term storage, store at -20 °C.
Antigen:
The antibody was developed by immunizing rabbits with synthetic peptides corresponding to amino acids 47-65 of the Anthrax LF protein (~90 kD).
Western Blot:
Western blot analysis of Anthrax LF in recombinant protein at 1:1000 dilution. Lane A shows the pre-bleed.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Background:
Anthrax PA (Protective Antigen) and Anthrax LF (Lethal Factor), along with Anthrax EF (Edema Factor), are toxins produced by Bacillus anthracis. All three of the proteins must be present for toxicity to occur. It begins with the binding of PA to its cell-surface receptor, ATR (Anthrax Toxin Receptor), at which point PA is cleaved and the resulting 63 kD carboxyl-terminal fragment binds to LF and EF. LF is a protease that is known to cleave members of the mitogen-activated protein kinase kinase (MAPKK) family(2).
Contents:
100µgs in 200µl PBS containing 0.2% gelatin and 0.05% sodium azide.
