- Description
- Specifications
Catalog #: CYT-333
Description:
Bone Morphogenetic Protein-7 Human Recombinant produced in E.Coli is a monomeric, non-glycosylated, polypeptide chain containing 139 amino acids and having a molecular mass of 15679.97 Dalton.
The BMP-7 is purified by proprietary chromatographic techniques.
Synonyms:
Osteogenic Protein 1, BMP-7.
Source:
Escherichia Coli.
Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Thr-Gly-Ser-Lys.
Applications:
1. Molecular standard (Western, ELISA) in studying secreted BMP-7.
2. Preparing antibodies for BMP-7 monomer.
3. Molecule standard in detecting secreted BMP-7 in reduced SDS-PAGE.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to briefly centrifuge the vial prior to opening to bring the contents to the bottom. Reconstitute in 20mM-100mM acetic acid at a concentration of 0.1-0.5mg per ml. Stock solutions should be apportioned into working aliquots and stored at <-20 °C. Further dilutions should be made in appropriate buffered solutions.
Formulation:
BMP-7 was lyophilized from a concentrated (1mg/ml) sterile solution containing 10mM sodium citrate pH=3.5.
Stability:
Lyophilized BMP-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution BMP 7 Human should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
