- Description
- Specifications
Catalog #: PRO-086
Description:
Cystatin A Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 98 amino acids and having a molecular mass of 11kDa.
The Cystatin A is purified by proprietary chromatographic techniques.
Synonyms:
Cystatin-A, Cystatin-AS, Stefin-A, CSTA, STF1, STFA.
Source:
Escherichia Coli.
Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ile-Pro-Gly-Gly.
Purity:
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Cystatin A in sterile 18M?-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
Cystatin A was lyophilized from a concentrated (1mg/ml) solution in 50mM Tris-HCl, pH 7.5 and 100mM NaCl.
Stability:
Lyophilized Cystatin A although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution CSTA should be stored at 4 °C between 2-7 days and for future use below -18 °C.
Please prevent freeze-thaw cycles.
Activity:
The IC50 value is < 3.4 nM, as measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
