- Description
- Specifications
Catalog #: ENZ-007
Description:
CDA Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 166 amino acids (1-146 a.a.) and having a molecular mass of 18.3kDa. The CDA is purified by proprietary chromatographic techniques.
Synonyms:
Cytidine deaminase, Cytidine aminohydrolase, CDA, CDD.
Source:
Escherichia Coli.
Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MAQKRPACTL KPECVQQLLV CSQEAKQSAY CPYSHFPVGA ALLTQEGRIF KGCNIENACY PLGICAERTA IQKAVSEGYK DFRAIAIASD MQDDFISPCG ACRQVMREFG TNWPVYMTKP DGTYIVMTVQ ELLPSSFGPE DLQKTQ.
Purity:
Greater than 90.0% as determined by SDS-PAGE.
Formulation:
The CDA solution (0.5 mg/ml) contains 20 mM Tris-HCl Buffer (pH 8.0), 1 mM DTT,
2mM EDTA, 100 mM NaCl and 40% Glycerol.
Stability:
Store at 4 °C if entire vial will be used within 2-4 weeks.
Store, frozen at -20 °C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Activity:
Specific activity :> 3.5unit/mg. One unit of activity was defined as the amount required to deaminate 1.0 umole of cytidine per min at pH 7.5 at 250C.
Physical Appearance:
Sterile Filtered colorless solution.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
