- Description
- Specifications
Catalog #: ENZ-289
Description:
Recombinant Saccharomyces Cerevisiae ICDH (NADP) derived from yeast host cells by using over-expression system, is full length same as designated ICD1 from Saccharomyces Cerevisiae. The N-terminal amino acid Phenylalanine residue next to Met is substituted with Alanine for overexpression.
The ICDH is purified by proprietary chromatographic techniques.
Synonyms:
Isocitrate dehydrogenase [NADP] cytoplasmic, EC 1.1.1.42, Cytosolic NADP-isocitrate dehydrogenase, Oxalosuccinate decarboxylase, IDH, NADP(+)-specific ICDH, IDP, PICD.
Source:
Yeast cells.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Formulation:
One ml of solution (1mg/340 µl) contains 0.075 mol/l KPO4, 50% Glycerol, pH 7.1.
Stability:
ICDH although stable at 15 °C for 1 week should be stored between 2 °C-8 °C.
For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA)
Please avoid freeze-thaw cycles.
Activity:
The specific activity was found to be 119 U/mg.
Physical Appearance:
Sterile Filtered clear solution.
Unit Definition:
One unit is defined as 1 µmol of NAD+ production per minute under the assay conditions (25 °C, pH 7.5).
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
