- Description
- Specifications
Catalog #: PRO-815
Description:
DKK1 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 184 amino acids with a molecular weight of 20.7 kDa though on SDS-PAGE migrates at about 28kDa due to the glycosilation.
The DKK1 is purified by proprietary chromatographic techniques.
Synonyms:
DKK-1, DKK1, HDKK1, HDKK-1, Dickkopf-1, Dickkopf-related protein 1, SK.
Source:
HEK293-F Cells.
Amino Acid Sequence:
CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA.
Purity:
Greater than 95.0% as determined by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized DKK1 in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The DKK1 protein was lyophilized from a 0.2 µm filtered solution containing no additives or preservatives.
Stability:
Lyophilized DKK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution DKK1 should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
IC50 = 0.4 to 1.2 nM, determined by the concentration dependent inhibition of human MMP-2 hydrolysis of a peptide substrate (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-(3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl)-Ala-Arg-NH2, under conditions in which recombinant pro-MMP-2 is activated, and the peptide substrate concentration is 5 µM.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
