- Description
- Specifications
Catalog #: CYT-120
Description:
FGF-18 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 173 amino acids and having a molecular mass of 20.1kDa.
The FGF-18 is purified by proprietary chromatographic techniques.
Synonyms:
Fibroblast growth factor 18, FGF-18, zFGF5, FGF18.
Source:
Escherichia Coli.
Amino Acid Sequence:
AEENVDFRIH VENQTRARDD VSRKQLRLYQ LYSRTSGKHI QVLGRRISAR GEDGDKYAQL LVETDTFGSQ VRIKGKETEF YLCMNRKGKL VGKPDGTSKE CVFIEKVLEN NYTALMSAKY SGWYVGFTKK GRPRKGPKTR ENQQDVHFMK RYPKGQPELQ KPFKYTTVTK RSR.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized FGF-18 in sterile 18M?-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
FGF-18 protein was lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Stability:
Lyophilized FGF-18 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution FGF-18 should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is < 0.5ng/ml, corresponding to a specific activity of > 2.0x106 units/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Patent Rights:
The Sale of recombinant Human FGF-18 by Denovo Biotechnology is prohibited in the following countries: U.S.A, Israel, Austria, Belgium, Switzerland, Germany, Denmark, Spain, Finland, France, United Kingdom, Greece, Ireland, Italy, Luxembourg, Monaco, The Netherlands, Portugal, Sweden, China, Hong Kong, India, Japan, Brazil, Canada, Mexico, Australia, Czech Republic, Hungary, Norway and Ukraine.
