- Description
- Specifications
Catalog #: CYT-415
Description:
Fibroblast Growth Factor-9 Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 207 amino acids and having a molecular mass of 23.4 kDa. The FGF-9 is purified by proprietary chromatographic techniques.
Synonyms:
GAF (Glia-activating factor), HBGF-9, MGC119914, MGC119915, FGF-9.
Source:
Escherichia coli.
Amino Acid Sequence:
APLGEVGNYF GVQDAVPFGN VPVLPVDSPV LLSDHLGQSE AGGLPRGPAV
TDLDHLKGIL RRRQLYCRTG FHLEIFPNGT IQGTRKDHSR FGILEFISIA
VGLVSIRGVD SGLYLGMNEK GELYGSEKLT QECVFREQFE ENWYNTYSSN
LYKHVDTGRR YYVALNKDGT PREGTRTKRH QKFTHFLPRP VDPDKVPELY
KDILSQS.
Purity:
Greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-9 Human Recombinant sterile 18M-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The sterile protein powder is lyophilized from 1mg/ml solution containing 1x PBS
Stability:
Lyophilized Fibroblast Growth Factor 9 Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution FGF-9 should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is <0.5 ng/ml, corresponding to a specific activity of 2,000,000IU/mg.
Physical Appearance:
Sterile Filtered white lyophilized powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY.They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
