- Description
- Specifications
Catalog #: CYT-613
Description:
Fibroblast Growth Factor-acidic Bovine (FGF-1) purified from Bovine Brain contains a 17 kDa and a 20 kDa polypeptide chain. The 17 kDa peptide is derived from the 20K peptide by restricted proteolysis. (See Jaye et al2). The FGF acidic is purified by proprietary chromatographic techniques.
Synonyms:
HBGF-1, ECGF-beta, FIBP, FGFIBP, FIBP-1, ECGF, ECGFA, GLIO703, FGF1, FGF-a.
Source:
Bovine Brain.
Purity:
Greater than 90%.
Solubility:
It is recommended to reconstitute the lyophilized aFGF in sterile 50mM Na2HPO4 pH-7, and 0.5% albumin. The Recommended concentration in cell culture: 1-20ng/ml.
Formulation:
Each 5 µg aFGF were lyophilized from 0.5ml solution containing 1mM sodium phosphate, pH 7 after filtration over a low binding membrane.
Stability:
Lyophilized aFGF although stable at room temperature for 2 weeks, should be stored desiccated below -18 °C. Upon reconstitution aFGF should be stored at 4 °C between 2-3 weeks and for future use below
-18 °C.
Please prevent freeze-thaw cycles.
Activity:
Stimulates growth of bovine capillary endothelial cells by 3-5 fold over 5% calf serum at 10-25ng/ml FGF and 0ug/ml heparin.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
