- Description
- Specifications
Catalog #: PRO-448
Source:
Human Plasma.
Purity:
Greater than 95.0% as determined by SDS-PAGE.
Solubility:
Purified fibronectin has a tendency to form insoluble aggregates upon reconstitution. We suggest reconstituting the 1mg Fibronectin with a chaotropic agent such as urea at room temperature at a concentration of 0.2mg/ml using sterile water.
Let stand 1-2 hours.
Formulation:
The Fibronectin was lyophilized from a concentrated 1mg/ml solution containing 0.05M Tris Cl pH-7.5 and 0.1M NaCl.
Stability:
Store the lyophilized fibronectin at 4 °C. Upon reconstitution fibronectin should be stored at 4 °C for 2 weeks and for future use below -18 °C.
Please prevent freeze-thaw cycles.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Cell Culture Applications:
Fibronectin is useful for the induction of cell attachment to a variety of surfaces including plastic and glass tissue culture labware, petri dishes, coverslips, microcarrier beads, etc. Fibronectin is useful for growth and maintenance of cells in low serum conditions. In general, the reconstituted fibronectin should be diluted with sterile physiological saline or serum-free medium to a concentration of 10-50ug/ml. The plates are treated with the solution, at room temperature 1-2 hours, the supernatant removed and the plates rinsed with fresh media or buffer. Growth media is then added and the coated plates seeded with a suspension of freshly harvested cells. Care should be taken to inactivate serum proteases used in the preparation of the cells, since fibronectin is very sensitive to proteolysis. Optimum concentration of fibronectin depends on the types of cell and the particular surface used. Generally, use 1-5ug/cm_ of growth surface.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
