- Description
- Specifications
Catalog #: CYT-331
Description:
Flt3-Ligand Human Recombinant produced in E.Coli is non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17605 Dalton.
Flt3-Ligand is purified by proprietary chromatographic techniques.
Synonyms:
Fms-related tyrosine kinase 3 ligand, FLK2, STK1, CD135, Stem Cell Tyrosine Kinase 1, FLT3LG, Flt3.
Source:
Escherichia Coli.
Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Gln-Asp-Cys-Ser.
Purity:
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Flt3-L in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The protein was lyophilized with no additives.
Stability:
Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution Flt3-Ligand should be stored at 4 °C between 2-7 days and for future use below -18 °C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The ED50 range=0.5-1.0 ng/ml corresponding to a specific activity of 1-2MUnits/mg, calculated by the dose-dependant stimulation of the proliferation of human OCMI-AML5 cells.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
