- Description
- Specifications
Catalog #: CYT-244
Description:
Hepatocyte Growth Factor Human Recombinant produced in Baculovirus is a heterodimer, non-glycosylated, polypeptide chain consisting an a-chain of 463 amino acids and b-chain of 234 having a total molecular mass of 78.0 KDa.
The HGF is purified by proprietary chromatographic techniques.
Synonyms:
Scatter Factor (SF), Hepatopoietin (HPTA), HGF, HGFB, F-TCF.
Source:
Insect cells.
Amino Acid Sequence:
Agrees with the sequence of native human HGF.
Purity:
Greater than 90.0% as determined by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor in sterile 18M-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. For further dilutions should be made into buffer containing 250mM NaCl.
Formulation:
The sterile protein powder (1 mg/ml) is lyophilized from a solution containing 50mM acetic acid.
Stability:
Lyophilized Hepatocyte Growth Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution HGF should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The activity was assayed for scattering activity in the MDCK cell assay. The ED50 for this effect is typically at 2.0-5.0 ng/ml (200,000-500,000U/mg).
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
