- Description
- Specifications
Catalog #: CYT-518
Description:
IGF-I Des(1-3) Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 67 amino acids (aa 4-70) and having a molecular mass of 7372 Dalton.
IGF-1 Des1-3 is purified by proprietary chromatographic techniques.
Synonyms:
Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF, Des(1-3), Des1-3, Des 1-3, Des (1-3), IGF-1 (4-70).
Source:
Escherichia Coli.
Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Leu-Cys-Gly-Ala.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized IGF-I Des(1-3) in 100mM Acetic acid not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The protein was lyophilized after extensive dialysis against 50mM acetic acid buffer.
Stability:
Lyophilized IGF-I des(1-3) although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IGF1 des-1-3 should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells (measured by 3H-thymidine uptake) is < 1ng/ml, corresponding to a specific activity of 1,000,000units/mg. For most in-vitro applications, IGF-I des1-3 exerts its biological activity in the concentration range of 0.2-20 ng/ml, corresponding to a specific activity of 50,000-5,000,000units/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
