- Description
- Specifications
Catalog #: CYT-265
Description:
Insulin-Like Growth Factor-II Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 67 amino acids and having a molecular mass of 7505 Dalton.
IGF-II is purified by proprietary chromatographic techniques.
Synonyms:
Somatomedin-A, IGF2, INSIGF, pp9974, C11orf43, FLJ22066, FLJ44734.
Source:
Escherichia Coli.
Amino Acid Sequence:
AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE.
Purity:
Greater than 97.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized IGF2 in sterile 18MΩ–cm H2O at a concentration of 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The protein was lyophilized with no additives.
Stability:
Lyophilized IGF-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IGFII should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The ED50, calculated by the dose-dependent induction of MCF-7 cell proliferation was found to be 0.85ng/ml corresponding to a Specific Activity of 1,176,470IU/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
