- Description
- Specifications
Catalog #: CYT-022
Description:
The LR3 is a long-term analog of human IGF-1, specifically designed and manufactured for mammalian cell culture to support large-scale manufacturing of recombinant biopharmaceuticals.
Recombinant Human Long R3 Insulin Like Growth Factor-1 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 83 amino acids and having a molecular mass of 9.1kDa.
Synonyms:
R3 IGF1, R3 IGF-1, R3IGF1, R3IGF-1, LONG IGF1, LONG IGF-1, LONG R3 IGF1, LONG R3IGF1, LONG R3 IGF-1, LONG R3IGF-1.
Source:
Escherichia Coli.
Amino Acid Sequence:
MFPAMPLSSLFVNGPRTLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIV DECCFRSCDLRRLEMYCAPLKPAKSA.
Purity:
Greater than 95.0% as determined by SDS-PAGE and HPLC.
Solubility:
It is recommended to reconstitute the lyophilized Long R3 IGF1 in sterile 18M-cm H2O at a concentration of 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
Lyophilized from a 0.2 µm filtered concentrated solution in 1xPBS.
Stability:
Lyophilized Long R3 IGF1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution the Long R3 IGF1 should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The ED50 as determined by the stimulation of protein synthesis in L6 myoblasts is less then 10 ng/ml, corresponding to a specific activity of 100,000 units/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
