- Description
- Specifications
Catalog #: CYT-520
Description:
Interferon-alpha 1a Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19.4 kDa.
The Interferon-alpha 1a contains Valine residue at position 114.
The IFN-a 1a is purified by proprietary chromatographic techniques.
Synonyms:
Interferon-alpha 1a, IFN-a 1a, IFN alpha 1a.
Source:
Escherichia Coli.
Amino Acid Sequence:
CDLPETHSLD NRRTLMLLAQ MSRISPSSCL MDRHDFGFPQ EEFDGNQFQK APAISVLHEL IQQIFNLFTT KDSSAAWDED LLDKFCTELY QQLNDLEACA MQEERVGETP LMNVDSILAV KKYFRRITLY LTEKKYSPCA WEVVRAEIMR SLSLSTNLQE RLRRKE.
Purity:
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized IFN alpha 1a in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
Lyophilized from a (1 mg/ml) solution in containing 1X PBS pH-7.4.
Stability:
Lyophilized Interferon alpha 1a although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IFN-alpha 1a should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The specific activity as determined in a viral resistance assay viral ressistance assay was found to be 100,000,000IU/ mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
