- Description
- Specifications
Catalog #: CYT-208
Description:
Interleukin-1 beta Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 153 amino acids and having a molecular mass of 17000 Dalton.
The IL-1b is purified by proprietary chromatographic techniques.
Synonyms:
Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta.
Source:
Escherichia Coli.
Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Val-Arg-Ser.
Purity:
Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Interleukin-1b in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing 50mM Phosphate buffer pH=7.1 and 150mM NaCl.
Stability:
Lyophilized Interleukin-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IL1b should be stored at 4 °C between 2-7 days and for future use below -18 °C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The specific activity as determined in the test of augmentation of lymphocyte proliferation assay using mouse thymus was found to be 200,000,000 IU/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Protein Content:
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.631 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-1 beta Recombinant as a Reference Standard.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Patent Rights:
The sale and/or commercial use of Recombinant Human IL1B is prohibited in the United States of America (U.S.A) and Japan.
References:
1.Title: Proinflammatory Cytokine IL-1b Stimulates IL-8 Synthesis in Mast Cells via a Leukotriene B4 Receptor 2-Linked Pathway, Contributing to Angiogenesis.
Publication: Published online before print March 1, 2010, doi: 10.4049/ jimmunol.0901735 The Journal of Immunology April 1, 2010 vol. 184 no. 7 3946-3954.
Link: http://www.jimmunol.org/content/184/7/3946.full
Application: Level of IL-8 mRNA was dramatically enhanced by IL-1b,HMC-1 cells were stimulated with IL-1b.
2.Title:Contribution of IL-1 to resistance to Streptococcus pneumoniae infection .
Publication: Int. Immunol. (2008) 20 (9): 1139-1146. doi: 10.1093/intimm/dxn071 First published online: July 1, 2008
Link:http://intimm.oxfordjournals.org/content/20/9/1139.full
