Interleukin-19 Mouse Recombinant - 10µg

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Catalog #: CYT-411Description:Interleukin-19 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids and having a molecular mass of 17722 Dalton.The IL-19 is purified by proprietary chromatographic techniques.Synonyms:Melanoma differentiation a ...Read more
Catalog # CYT-411 $225.00 each


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  • Description
  • Specifications

Catalog #: CYT-411

Description:
Interleukin-19 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids and having a molecular mass of 17722 Dalton.
The IL-19 is purified by proprietary chromatographic techniques.

Synonyms:
Melanoma differentiation association like protein, MDA1, NG.1, ZMDA1, IL-10C, IL-19.

Source:
Escherichia Coli.

Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met- Leu-Arg-Arg-Cys.

Purity:
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility:
It is recommended to reconstitute the lyophilized Interleukin-19 in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Formulation:
Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.

Stability:
Lyophilized Interleukin-19 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IL19 should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Activity:
The biological activity was determined by measuring the dose-dependent activation of STAT3 in human epidermal keratinocytes (HEK2a cells). Significant STAT3 activation is observed with >100ng/ml corresponding to a Specific Activity of 10,000IU/mg of IL-19 recombinant mouse.

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Protein Content:
Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-19 as a Reference Standard.

Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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