- Description
- Specifications
Catalog #: CYT-382
Description:
Interleukin-2 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 17kDa.
The IL-2 is purified by proprietary chromatographic techniques.
Synonyms:
T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2.
Source:
Escherichia Coli.
Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Ser.
Purity:
Greater than 95.0% as determined by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Interleukin 2 in sterile 100mM AcOH not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The protein (1mg/ml) was lyophilized without any additives.
Stability:
Lyophilized Interleukin-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IL2 should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is 0.1-0.4 ng/ml corresponding to a specific activity of 2,500,000-10,000,000 units/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.535 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
References:
Title:Functional Gap Junctions Facilitate Melanoma Antigen Transfer and Cross-Presentation between Human Dendritic Cells.
Publication:The Journal of Immunology June 1, 2007 vol. 178 no. 11 6949-6957
Link:http://www.jimmunol.org/content/178/11/6949.full
