- Description
- Specifications
Catalog #: CYT-158
Description:
IL36A Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 153 amino acids (aa 6-158) and having a molecular mass of 17.0kDa.
The IL36A is purified by proprietary chromatographic techniques.
Synonyms:
Interleukin 36 alpha, FIL1E, IL1F6, FIL1, IL1(EPSILON), interleukin 1 family member 6 (epsilon), MGC129552, MGC129553.
Source:
Escherichia Coli.
Amino Acid Sequence:
KIDTPQQGSI QDINHRVWVL QDQTLIAVPR KDRMSPVTIA LISCRHVETL EKDRGNPIYL GLNGLNLCLM CAKVGDQPTL QLKEKDIMDL YNQPEPVKSF LFYHSQSGRN STFESVAFPG WFIAVSSEGG CPLILTQELG KANTTDFGLT MLF
Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized IL36A in sterile 18M?-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
Lyophilized from a 0.2 µm filtered concentrated solution in 20mM Tris-HCl, 150mM NaCl, 0.1% Tween 20, pH 8.0.
Stability:
Lyophilized IL36A Human although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IL36A should be stored at 4 °C between 2-7 days and for future use below -18 °C.
Please prevent freeze-thaw cycles.
Activity:
Fully biologically active when compared to standard. The ED50 as measured by its ability to induce IL-8 secretion in human preadipocytes is less than 10ng/ml, corresponding to a Specific Activity of 100,000 IU/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
