- Description
- Specifications
Catalog #: ENZ-319
Description:
Deoxyribonuclease I Human Recombinant produced in CHO is a glycosylated, polypeptide chain containing 260 amino acids and having a total molecular mass of 37,000 Dalton with a molecular formula of C1321H1995O396S9.
DNase is purified by proprietary chromatographic techniques.
Synonyms:
EC 3.1.21.1, Deoxyribonuclease I, DNase I, DNL1, DRNI, FLJ38093, DNASE1.
Source:
Chinese Hamster Ovary Cells.
Formulation:
Each mg contains 150 µg calcium chloride dehydrate and 8.77 mg NaCl.
Stability:
2 years when stored at 4 °C, three weeks at 15 °C, pH-6.3.
Activity:
1000IU/1mg.
Physical Appearance:
Sterile liquid colorless solution at a concentration of 1mg/ml.
Unit Definition:
Dnase is generally assayed according to the photometric method developed by Kunitz. One Dnase unit results in an increase in absorbance at 260nm of 0.001/minute at 25 °C when acting upon highly polymerized solution of DNA at pH-5. Also 0.005 Kunitz unit digests 1 µgof lambda phage DNA in 10 minutes at 37 °C in50mM Tris, 1mMMg++, pH 7.8 in a 50ul reaction.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
