- Description
- Specifications
Catalog #: PRO-303
Description:
PCNA Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 29,794 Dalton.
PCNA is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
Synonyms:
Proliferating cell nuclear antigen, PCNA, Cyclin, MGC8367.
Source:
Sf9 insect cells.
Applications:
Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.
Purity:
Greater than 95% as determined by SDS-PAGE.
Formulation:
Recombinant Human PCNA is supplied in16mM HEPES buffer pH-8, 400mM NaCl, and 20% glycerol.
Physical Appearance:
Sterile Filtered colorless solution.
Coating Concentration:
0.5-1.1 µg/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.
Immunological Functions:
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot analysis with validated SLE patient sera.
Storage:
Store at 4 °C if entire vial will be used within 2-4 weeks.
Store, frozen at -20 °C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
