- Description
- Specifications
Catalog #: ENZ-322
Description:
The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.
Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity.
Synonyms:
EC 3.1.3.1, IAP, AP.
Source:
Calf Intestine.
Purity:
95% pure by Gel Filtration.
Formulation:
50% glycerol, 5mM MgCl2, 0.1mM ZnCl2 and 5mM TRIS, pH 7.0.
Stability:
AP should be stored at 4 °C.
Please Do-Not freeze.
Activity:
>1000 U/mg (pH 9.6, 25 °C and 0.025M glycin, 10% glycerol as buffer.
Physical Appearance:
Sterile Filtered clear colorless solution.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
