- Description
- Specifications
Catalog #: CYT-595
Description:
Extra Cellular Domain Prolactin Receptor Human Recombinant ?produced in E.Coli is a non-glycosylated, Polypeptide chain containsing 210 amino acids and having a molecular mass of 23.97 kDa.
The Prolactin Receptor is purified by proprietary chromatographic techniques according to Bignon et al. (1994) JBC 269; 3318-24 and tested according to Gertler et al. (1996) JBC 271; 24482-91.
Synonyms:
PRL-R, hPRLrI.
Source:
Escherichia Coli.
Amino Acid Sequence:
The sequence of the first six N-terminal amino acids was determined to be Ala-Gly-Lys-Pro-Glu-Ile.
Purity:
Greater than 97.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
(c) Gel filtration at pH 8 under non denaturative conditions.
Solubility:
It is recommended to reconstitute the lyophilized PRLR in sterile 18M-cm H2O not less than 100 µg/ml and not more than 1 mg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The Prolactin Receptor was lyophilized from a concentrated (0.4mg/ml) solution with 0.0045mM NaHCO3.
Stability:
Lyophilized PRL-R although stable at room temperature for 1-2 weeks, should be stored desiccated below -18 °C or preferably even at -80 °C to prevent dimer formation. Upon reconstitution PRL-R should be stored sterile at 4 °C between 2-7 days and for future use below -18 °C. For long term storage at 4 °C it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles as they cause oligomerization of the protein.
Activity:
Activity is determined by the dose-dependant inhibition of Prolactin stimuled proliferation of Nb2 cells and by high affinity binding of ovine Prolactin and other lactogenic hormones in 1:1 molar ratio.
Physical Appearance:
Sterile filtered white lyophilized powder.
Protein Content:
UV spectroscopy at 280 nm using the absorbency value of 2.63 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
