- Description
- Specifications
Catalog #: PKA-317
Description:
CSRC Human Recombinant produced in Sf9 cells is a glycosylated, polypeptide chain having a molecular mass of 60 KD.
CSRC is purified by proprietary chromatographic techniques.
Synonyms:
Tyrosine-protein kinase CSK, EC 2.7.10.2, C-SRC kinase, Protein-tyrosine kinase CYL, CSK, C-SRC.
Source:
Sf9 Insect Cells.
Purity:
Greater than 95% as determined by SDS-PAGE.
Formulation:
C-SRC is supplied at a concentration of 0.29mg/ml in 50mM Tris, pH 7.6, 0.15 M NaCl, 0.27M sucrose, 1mM DTT, 0.1mM EGTA, 0.2mM PMSF, 1mM benzamidine, 5mM NaF, 10mM beta-glycerol phosphate, 0.1mM sodium vanadate and 0.03% Brij-35.
Activity:
750 units/mg. One unit of C-SRC activity is equal to 1 nmol of phosphate transferred to a peptide corresponding to p34cdc2 (6-20) per minute at 30 °C with a final ATP concentration of 100 µM.
Recombinant human active C-SRC also phosphorylates STAT, p34cdc2, gastrin, and cortactin.
Note:
Kinase activity may vary depending on the substrate and reaction conditions.
Physical Appearance:
Sterile Filtered clear solution.
Storage:
Store at 4 °C if entire vial will be used within 1-2 weeks. Store frozen at -20 °C for longer periods of time. Avoid multiple freeze-thaw cycles.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
