- Description
- Specifications
Catalog #: PRO-445
Description:
Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein (lacking 66 internal amino acids outside the known epitope-containing areas) fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa (U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight and the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis).
Synonyms:
U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP.
Source:
Escherichia Coli.
Applications:
Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera (Mixed Connective Tissue Disease).
Purity:
Greater than 90% as determined by SDS-PAGE.
Formulation:
U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA and 0.02% SDS.
Physical Appearance:
Sterile Filtered clear solution.
Coating Concentration:
0.3-0.6 µg/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.
Immunological Functions:
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
Storage:
Store at 4 °C if entire vial will be used within 2-4 weeks.
Store, frozen at -20 °C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
