- Description
- Specifications
Catalog #: CYT-302
Description:
Thrombopoietin Human Recombinant produced in E.Coli is a single, non-glycosylated soluble polypeptide chain containing 174 amino acids and having a molecular mass of 18.6kDa.
The TPO is purified by proprietary chromatographic techniques.
Synonyms:
Megakaryocyte colony-stimulating factor, Myeloproliferative leukemia virus oncogene ligand, C-mpl ligand, ML, Megakaryocyte growth and development factor, MGDF, TPO, MKCSF, MPLLG, MGC163194, THPO.
Source:
Escherichia Coli.
Amino Acid Sequence:
SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL.
Purity:
Greater than 98.0% as determined by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Thrombopoietin in sterile 18M?cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
Lyophilized from 10mM Na2PO4, pH 8.0.
Stability:
Lyophilized Thrombopoietin although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution TPO Human should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The ED50 as determined by the dose-dependant stimulation of MO7e cells is < 1 ng/ml, corresponding to a specific activity of 1,000,000units/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
