- Description
- Specifications
Catalog #: CYT-716
Description:
TGFB1 Human Recombinant produced in Human 293 cells is a homodimeric polypeptide chain containing 2 x 112 amino acids and having a total molecular mass of 25kDa.
The TGFB1 is purified by proprietary chromatographic techniques.
Synonyms:
Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1, LAP, TGFB1.
Source:
Human 293 cells.
Amino Acid Sequence:
ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS.
Purity:
Greater than 98.0% as determined by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized TGFB1 in sterile 5mM HCl & 50ug/ml BSA at a concentration of 0.1mg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
Lyophilized from a concentrated (1mg/ml) solution containing 10mM HCl & 50ug BSA per mg/ml of protein.
Stability:
Lyophilized TGFB1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution TGFB1 Human should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The ED50, as measured by the dose-dependent inhibition of IL-4-induced proliferation of mouse HT-2 cells is typically 0.1-0.15ng/ml.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
References:
1.Title:Imatinib attenuates skeletal muscle dystrophy in mdx mice .
Publication:Published online before print March 16, 2009, doi: 10.1096/fj.09-129833 August 2009 The FASEB Journal vol. 23 no. 8 2539-2548 .
Link:http://www.fasebj.org/content/23/8/2539.full
2.Title:Characterization of Non-Specific Cytotoxic Cell Receptor Protein 1: A New Member of the Lectin-Type Subfamily of F-Box Proteins.
Publication:Kallio H, Tolvanen M, J?nis J, Pan P-w, Laurila E, et al. (2011) Characterization of Non-Specific Cytotoxic Cell Receptor Protein 1: A New Member of the Lectin-Type Subfamily of F-Box Proteins. PLoS ONE 6(11): e27152. doi:10.1371/journal.pone.0027152
Link:http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0027152
