- Description
- Specifications
Catalog #: CYT-628
Description:
VEGFB Human Recombinant produced in E.Coli is a double, non-glycosylated, polypeptide chain containing 207 amino acids (22-207) and having a molecular mass of 21.6 kDa.
The VEGFB is fused to a 20 amino acid His Tag at N-terminus & purified by proprietary chromatographic techniques.
Synonyms:
Vascular endothelial growth factor B, VEGF-B, VRF, VEGFL, VEGF-related factor, VEGFB.
Source:
Escherichia Coli.
Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MPVSQPDAPG HQRKVVSWID VYTRATCQPR EVVVPLTVEL MGTVAKQLVP SCVTVQRCGG CCPDDGLECV PTGQHQVRMQ ILMIRYPSSQ LGEMSLEEHS QCECRPKKKD SAVKPDRAAT PHHRPQPRSV PGWDSAPGAP SPADITHPTP APGPSAHAAP STTSALTPGP AAAAADAAAS SVAKGGA.
Purity:
Greater than 90.0% as determined by SDS-PAGE.
Formulation:
The VEGFB protein solution contains 20mM Tris-HCl buffer pH 7.5 & 10% glycerol.
Stability:
Store at 4 °C if entire vial will be used within 2-4 weeks. Store, frozen at -20 °C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Activity:
Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 for this effect is < 1ng/ml corresponding to a specific activity of less than 1,000,000units/mg.
Physical Appearance:
Sterile Filtered colorless liquid formulation.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
